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Cayman’s SARS-CoV-2 Spike S1 RBD-ACE2 Binding Cellular Imaging Assay Kit provides a sensitive and unique imaging platform for identifying novel modulators of SARS-CoV-2 spike S1 RBD and ACE2 binding. This assay uses Cayman’s proprietary reverse transfection platform to express human ACE2 on the surface of HEK293T/17 cells for binding by a recombinant rabbit Fc-tagged SARS-CoV-2 spike S1 RBD protein as a model of SARS-CoV-2 cellular infection.{55525} Binding of the spike S1 RBD to ACE2 is detected with a DyLight™ 550-conjugated anti-rabbit Fc antibody by fluorescence imaging. Inhibition of spike S1 RBD binding to ACE2 results in decreased fluorescence. A positive control neutralizing antibody is included to inhibit the binding between the SARS-CoV-2 spike S1 RBD and ACE2.
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Bacillus calmette-guerin substrain tice live antigen is a vaccine containing attenuated live culture preparation of the Bacillus of Calmette and Guerin (BCG) strain of Mycobacterium bovis for percutan
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
The Meso Scale Discovery V-PLEX SARS-CoV-2 Panel 2 (2G) Mouse IgA Kit is an electrochemiluminescence immunoassay that detects IgA antibodies to SARS-CoV-2 in mouse serum and plasma samples The assay uses plates coated with SARS-CoV-2 spike protein S1 and nucleocapsid protein antigens to capture anti-SARS-CoV-2 antibodies in the sample Bound antibodies are detected using an anti-mouse IgA detection antibody conjugated to an electrochemiluminescent label The kit includes reagents for 25 tests and can quantitatively measure IgA antibodies with high sensitivity and specificity
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A DNA sequence encoding the glycoprotein G extracellular domain (Asn 66-Arg 297) of human respiratory syncytial virus A (95 % homologous with strain rsb1734) (P27022-1) was expressed, with a C-terminal polyhistidine tag.
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